Mission

HOPE is developed at the Centre for Molecular and Biomolecular Informatics CMBI at Radboud University in Nijmegen.

Our mission is to build an automatic mutant analysis server that can provide insight into the structural effects of a mutation. HOPE is aimed at users in the (bio)medical field who wish to visualize and understand their mutation of interest. We hope that these new insights can be used directly in a publication and/or for intelligent design of further experiments. The fact that a 3D analysis of a mutated protein structure can contribute to a wide range of research fields is underlined by earlier projects in which the structural analysis was performed manually. (Descriptions and publications of these projects can be found here.

Method

HOPE goes through a complex process to analyse the mutant of interest. More information about this process can be found on the method page.

We tested Project HOPE by running a number of previously analyzed cases. We compared HOPE's results with the results of our manual analysis and with the results of the well-know PolyPhen and SIFT methods. A page with a summary of these results can be found on here.

Citing HOPE

Please use the following citation when referencing the results in your report:

Protein structure analysis of mutations causing inheritable diseases. An e-Science approach with life scientist friendly interfaces.

BMC Bioinformatics. 2010 Nov 8;11(1):548. DOI: 10.1186/1471-2105-11-548. PubMed: 21059217.

Informal Notice

Please express your gratitude for this free material by citing the above article.

The material provided is complete, correct to the best of our knowledge, and holds all that is needed to start building your own HOPE server. If you do so, please provide a link to the HOPE server. If you want to get a flying start by getting access to the HOPE decision tree, feel free to contact us.