WHAT IF Check report

This file was created 2011-12-13 from WHAT_CHECK output by a conversion script. If you are new to WHAT_CHECK, please study the pdbreport pages. There also exists a legend to the output.

Please note that you are looking at an abridged version of the output (all checks that gave normal results have been removed from this report). You can have a look at the Full report instead.

Verification log for pdb1bsi.ent

Coordinate problems, unexpected atoms, B-factor and occupancy checks

Warning: What type of B-factor?

WHAT IF does not yet know well how to cope with B-factors in case TLS has been used. It simply assumes that the B-factor listed on the ATOM and HETATM cards are the total B-factors. When TLS refinement is used that assumption sometimes is not correct. TLS seems not mentioned in the header of the PDB file. But anyway, if WHAT IF complains about your B-factors, and you think that they are OK, then check for TLS related B-factor problems first.

Obviously, the temperature at which the X-ray data was collected has some importance too:

Crystal temperature (K) :298.000

Geometric checks

Warning: Unusual bond angles

The bond angles listed in the table below were found to deviate more than 4 sigma from standard bond angles (both standard values and sigma for protein residues have been taken from Engh and Huber [REF], for DNA/RNA from Parkinson et al [REF]). In the table below for each strange angle the bond angle and the number of standard deviations it differs from the standard values is given. Please note that disulphide bridges are neglected. Atoms starting with "-" belong to the previous residue in the sequence.

 161 ARG   ( 161-)  A      CB   CG   CD  105.25   -4.4
 237 LEU   ( 237-)  A      N    CA   C    97.33   -5.0
 239 GLY   ( 239-)  A      N    CA   C   129.18    5.8

Error: Tau angle problems

The side chains of the residues listed in the table below contain a tau angle (N-Calpha-C) that was found to deviate from te expected value by more than 4.0 times the expected standard deviation. The number in the table is the number of standard deviations this RMS value deviates from the expected value.

 237 LEU   ( 237-)  A    5.65
 239 GLY   ( 239-)  A    5.60
 310 ALA   ( 310-)  A    4.96
 318 ALA   ( 318-)  A    4.72
 164 GLY   ( 164-)  A    4.64
 162 LEU   ( 162-)  A    4.25

Torsion-related checks

Warning: Torsion angle evaluation shows unusual residues

The residues listed in the table below contain bad or abnormal torsion angles.

These scores give an impression of how `normal' the torsion angles in protein residues are. All torsion angles except omega are used for calculating a `normality' score. Average values and standard deviations were obtained from the residues in the WHAT IF database. These are used to calculate Z-scores. A residue with a Z-score of below -2.0 is poor, and a score of less than -3.0 is worrying. For such residues more than one torsion angle is in a highly unlikely position.

 407 THR   ( 407-)  A    -2.6
 142 LYS   ( 142-)  A    -2.4
 124 ARG   ( 124-)  A    -2.3
  45 PRO   (  45-)  A    -2.2
  56 ARG   (  56-)  A    -2.2
 459 ASN   ( 459-)  A    -2.2
 347 GLN   ( 347-)  A    -2.2
 437 SER   ( 437-)  A    -2.1
  35 LYS   (  35-)  A    -2.1
 438 LEU   ( 438-)  A    -2.1
 341 SER   ( 341-)  A    -2.1
 444 LEU   ( 444-)  A    -2.1
  69 LEU   (  69-)  A    -2.0
 440 LEU   ( 440-)  A    -2.0

Warning: Backbone evaluation reveals unusual conformations

The residues listed in the table below have abnormal backbone torsion angles.

Residues with `forbidden' phi-psi combinations are listed, as well as residues with unusual omega angles (deviating by more than 3 sigma from the normal value). Please note that it is normal if about 5 percent of the residues is listed here as having unusual phi-psi combinations.

   5 ASN   (   5-)  A  Poor phi/psi
  18 GLU   (  18-)  A  Poor phi/psi
  53 ASN   (  53-)  A  PRO omega poor
 102 MET   ( 102-)  A  Poor phi/psi
 124 ARG   ( 124-)  A  Poor phi/psi
 129 VAL   ( 129-)  A  PRO omega poor
 198 ALA   ( 198-)  A  Poor phi/psi
 221 TRP   ( 221-)  A  Poor phi/psi
 237 LEU   ( 237-)  A  Poor phi/psi
 268 LYS   ( 268-)  A  Poor phi/psi
 364 ASN   ( 364-)  A  Poor phi/psi
 376 THR   ( 376-)  A  Poor phi/psi
 380 ASN   ( 380-)  A  Poor phi/psi
 381 ASP   ( 381-)  A  Poor phi/psi
 414 SER   ( 414-)  A  Poor phi/psi
 486 PRO   ( 486-)  A  Poor phi/psi
 chi-1/chi-2 correlation Z-score : -2.300

Warning: Unusual backbone conformations

For the residues listed in the table below, the backbone formed by itself and two neighbouring residues on either side is in a conformation that is not seen very often in the database of solved protein structures. The number given in the table is the number of similar backbone conformations in the database with the same amino acid in the centre.

For this check, backbone conformations are compared with database structures using C-alpha superpositions with some restraints on the backbone oxygen positions.

A residue mentioned in the table can be part of a strange loop, or there might be something wrong with it or its directly surrounding residues. There are a few of these in every protein, but in any case it is worth looking at!

   5 ASN   (   5-)  A      0
   8 GLN   (   8-)  A      0
  12 SER   (  12-)  A      0
  17 PHE   (  17-)  A      0
  18 GLU   (  18-)  A      0
  19 TRP   (  19-)  A      0
  21 TRP   (  21-)  A      0
  30 ARG   (  30-)  A      0
  31 TYR   (  31-)  A      0
  32 LEU   (  32-)  A      0
  45 PRO   (  45-)  A      0
  52 TYR   (  52-)  A      0
  53 ASN   (  53-)  A      0
  54 PRO   (  54-)  A      0
  55 PHE   (  55-)  A      0
  56 ARG   (  56-)  A      0
  59 TRP   (  59-)  A      0
  62 TYR   (  62-)  A      0
  63 GLN   (  63-)  A      0
  64 PRO   (  64-)  A      0
  66 SER   (  66-)  A      0
  67 TYR   (  67-)  A      0
  69 LEU   (  69-)  A      0
  70 CYS   (  70-)  A      0
  73 SER   (  73-)  A      0
And so on for a total of 225 lines.

Warning: Omega angles too tightly restrained

The omega angles for trans-peptide bonds in a structure are expected to give a gaussian distribution with the average around +178 degrees and a standard deviation around 5.5 degrees. These expected values were obtained from very accurately determined structures. Many protein structures are too tightly restrained. This seems to be the case with the current structure too, as the observed standard deviation is below 4.0 degrees.

Standard deviation of omega values : 2.530

Warning: Backbone oxygen evaluation

The residues listed in the table below have an unusual backbone oxygen position.

For each of the residues in the structure, a search was performed to find 5-residue stretches in the WHAT IF database with superposable C-alpha coordinates, and some restraining on the neighbouring backbone oxygens.

In the following table the RMS distance between the backbone oxygen positions of these matching structures in the database and the position of the backbone oxygen atom in the current residue is given. If this number is larger than 1.5 a significant number of structures in the database show an alternative position for the backbone oxygen. If the number is larger than 2.0 most matching backbone fragments in the database have the peptide plane flipped. A manual check needs to be performed to assess whether the experimental data can support that alternative as well. The number in the last column is the number of database hits (maximum 80) used in the calculation. It is "normal" that some glycine residues show up in this list, but they are still worth checking!

 308 GLY   ( 308-)  A   1.87   12

Warning: Unusual PRO puckering phases

The proline residues listed in the table below have a puckering phase that is not expected to occur in protein structures. Puckering parameters were calculated by the method of Cremer and Pople [REF]. Normal PRO rings approximately show a so-called envelope conformation with the C-gamma atom above the plane of the ring (phi=+72 degrees), or a half-chair conformation with C-gamma below and C-beta above the plane of the ring (phi=-90 degrees). If phi deviates strongly from these values, this is indicative of a very strange conformation for a PRO residue, and definitely requires a manual check of the data. Be aware that this is a warning with a low confidence level. See: Who checks the checkers? Four validation tools applied to eight atomic resolution structures [REF].

  45 PRO   (  45-)  A   -60.7 half-chair C-beta/C-alpha (-54 degrees)
 130 PRO   ( 130-)  A   -38.5 envelop C-alpha (-36 degrees)
 374 PRO   ( 374-)  A  -128.5 half-chair C-delta/C-gamma (-126 degrees)
 486 PRO   ( 486-)  A   -63.4 envelop C-beta (-72 degrees)

Bump checks

Error: Abnormally short interatomic distances

The pairs of atoms listed in the table below have an unusually short interactomic distance; each bump is listed in only one direction.

The contact distances of all atom pairs have been checked. Two atoms are said to `bump' if they are closer than the sum of their Van der Waals radii minus 0.40 Angstrom. For hydrogen bonded pairs a tolerance of 0.55 Angstrom is used. The first number in the table tells you how much shorter that specific contact is than the acceptable limit. The second distance is the distance between the centres of the two atoms. Although we believe that two water atoms at 2.4 A distance are too close, we only report water pairs that are closer than this rather short distance.

The last text-item on each line represents the status of the atom pair. If the final column contains the text 'HB', the bump criterion was relaxed because there could be a hydrogen bond. Similarly relaxed criteria are used for 1-3 and 1-4 interactions (listed as 'B2' and 'B3', respectively). BL indicates that the B-factors of the clashing atoms have a low B-factor thereby making this clash even more worrisome. INTRA and INTER indicate whether the clashes are between atoms in the same asymmetric unit, or atoms in symmetry related asymmetric units, respectively.

 349 GLN   ( 349-)  A      O   <->  352 ASN   ( 352-)  A      ND2    0.24    2.46  INTRA
 218 ASN   ( 218-)  A      ND2 <->  220 ASN   ( 220-)  A      CB     0.16    2.94  INTRA BL
 494 SER   ( 494-)  A      N   <->  495 LYS   ( 495-)  A      N      0.14    2.46  INTRA BL
 185 HIS   ( 185-)  A      ND1 <->  501 HOH   ( 669 )  A      O      0.13    2.57  INTRA
 218 ASN   ( 218-)  A      ND2 <->  220 ASN   ( 220-)  A      N      0.09    2.76  INTRA BL
   7 GLN   (   7-)  A      N   <->  501 HOH   ( 508 )  A      O      0.08    2.62  INTRA
 170 LEU   ( 170-)  A      CD1 <->  202 MET   ( 202-)  A      SD     0.08    3.32  INTRA BL
 201 HIS   ( 201-)  A      NE2 <->  501 HOH   ( 578 )  A      O      0.08    2.62  INTRA
 331 HIS   ( 331-)  A      NE2 <->  501 HOH   ( 577 )  A      O      0.07    2.63  INTRA
 480 SER   ( 480-)  A      C   <->  482 SER   ( 482-)  A      N      0.07    2.83  INTRA
 331 HIS   ( 331-)  A      O   <->  398 ARG   ( 398-)  A      NH1    0.06    2.64  INTRA BL
 373 ASN   ( 373-)  A      O   <->  376 THR   ( 376-)  A      N      0.06    2.64  INTRA
 347 GLN   ( 347-)  A      OE1 <->  349 GLN   ( 349-)  A      NE2    0.05    2.65  INTRA BF
 267 ARG   ( 267-)  A      N   <->  268 LYS   ( 268-)  A      N      0.04    2.56  INTRA BL
 415 ASN   ( 415-)  A      ND2 <->  501 HOH   ( 548 )  A      O      0.04    2.66  INTRA BL
 347 GLN   ( 347-)  A      NE2 <->  349 GLN   ( 349-)  A      NE2    0.04    2.81  INTRA BF
  33 ALA   (  33-)  A      N   <->   34 PRO   (  34-)  A      CD     0.04    2.96  INTRA BL
   2 TYR   (   2-)  A      N   <->  501 HOH   ( 599 )  A      O      0.03    2.67  INTRA
 307 ALA   ( 307-)  A      N   <->  501 HOH   ( 661 )  A      O      0.03    2.67  INTRA BF
 373 ASN   ( 373-)  A      N   <->  377 THR   ( 377-)  A      O      0.02    2.68  INTRA
 258 TYR   ( 258-)  A      CE2 <->  280 TRP   ( 280-)  A      NE1    0.01    3.09  INTRA BL
 149 GLU   ( 149-)  A      N   <->  156 GLN   ( 156-)  A      OE1    0.01    2.69  INTRA BL
 347 GLN   ( 347-)  A      CD  <->  349 GLN   ( 349-)  A      NE2    0.01    3.09  INTRA BF
 407 THR   ( 407-)  A      O   <->  420 GLY   ( 420-)  A      N      0.01    2.69  INTRA BL
 345 PRO   ( 345-)  A      O   <->  355 ASN   ( 355-)  A      ND2    0.01    2.69  INTRA
 152 ASN   ( 152-)  A      N   <->  153 ASP   ( 153-)  A      N      0.01    2.59  INTRA B3

Packing, accessibility and threading

Warning: Abnormal packing environment for some residues

The residues listed in the table below have an unusual packing environment.

The packing environment of the residues is compared with the average packing environment for all residues of the same type in good PDB files. A low packing score can indicate one of several things: Poor packing, misthreading of the sequence through the density, crystal contacts, contacts with a co-factor, or the residue is part of the active site. It is not uncommon to see a few of these, but in any case this requires further inspection of the residue.

  72 ARG   (  72-)  A      -7.01
 343 ARG   ( 343-)  A      -6.19
   2 TYR   (   2-)  A      -6.13
  52 TYR   (  52-)  A      -5.94
   8 GLN   (   8-)  A      -5.89
 142 LYS   ( 142-)  A      -5.77
   7 GLN   (   7-)  A      -5.65
 237 LEU   ( 237-)  A      -5.62
 269 TRP   ( 269-)  A      -5.50
 284 TRP   ( 284-)  A      -5.47
 279 ASN   ( 279-)  A      -5.34
  30 ARG   (  30-)  A      -5.28
 302 GLN   ( 302-)  A      -5.27
 270 ASN   ( 270-)  A      -5.27
 303 ARG   ( 303-)  A      -5.24
  53 ASN   (  53-)  A      -5.21

Warning: Abnormal packing environment for sequential residues

A stretch of at least three sequential residues with a questionable packing environment was found. This could indicate that these residues are part of a strange loop. It might also be an indication of misthreading in the density. However, it can also indicate that one or more residues in this stretch have other problems such as, for example, missing atoms, very weird angles or bond lengths, etc.

The table below lists the first and last residue in each stretch found, as well as the average residue score of the series.

 269 TRP   ( 269-)  A       271 - GLY    271- ( A)         -4.99

Warning: Low packing Z-score for some residues

The residues listed in the table below have an unusual packing environment according to the 2nd generation packing check. The score listed in the table is a packing normality Z-score: positive means better than average, negative means worse than average. Only residues scoring less than -2.50 are listed here. These are the unusual residues in the structure, so it will be interesting to take a special look at them.

  17 PHE   (  17-)  A   -2.51

Warning: Abnormal packing Z-score for sequential residues

A stretch of at least four sequential residues with a 2nd generation packing Z-score below -1.75 was found. This could indicate that these residues are part of a strange loop or that the residues in this range are incomplete, but it might also be an indication of misthreading.

The table below lists the first and last residue in each stretch found, as well as the average residue Z-score of the series.

 355 ASN   ( 355-)  A     -  358 VAL   ( 358-)  A        -1.55

Water, ion, and hydrogenbond related checks

Warning: Water molecules need moving

The water molecules listed in the table below were found to be significantly closer to a symmetry related non-water molecule than to the ones given in the coordinate file. For optimal viewing convenience revised coordinates for these water molecules should be given.

The number in brackets is the identifier of the water molecule in the input file. Suggested coordinates are also given in the table. Please note that alternative conformations for protein residues are not taken into account for this calculation. If you are using WHAT IF / WHAT-CHECK interactively, then the moved waters can be found in PDB format in the file: MOVEDH2O.pdb.

 501 HOH   ( 674 )  A      O      4.89   70.43   60.67

Error: Water molecules without hydrogen bonds

The water molecules listed in the table below do not form any hydrogen bonds, neither with the protein or DNA/RNA, nor with other water molecules. This is a strong indication of a refinement problem. The last number on each line is the identifier of the water molecule in the input file.

 501 HOH   ( 580 )  A      O
 501 HOH   ( 660 )  A      O
 501 HOH   ( 664 )  A      O
 501 HOH   ( 702 )  A      O
Bound group on Asn; dont flip  461 ASN  ( 461-) A
Bound to:  497 NAG  ( 497-) A
Marked this atom as acceptor  500  CL  ( 499-) A     CL
Strange metal coordination for HIS 201

Error: HIS, ASN, GLN side chain flips

Listed here are Histidine, Asparagine or Glutamine residues for which the orientation determined from hydrogen bonding analysis are different from the assignment given in the input. Either they could form energetically more favourable hydrogen bonds if the terminal group was rotated by 180 degrees, or there is no assignment in the input file (atom type 'A') but an assignment could be made. Be aware, though, that if the topology could not be determined for one or more ligands, then this option will make errors.

 105 ASN   ( 105-)  A
 150 ASN   ( 150-)  A
 218 ASN   ( 218-)  A
 270 ASN   ( 270-)  A
 305 HIS   ( 305-)  A
 415 ASN   ( 415-)  A

Warning: Buried unsatisfied hydrogen bond donors

The buried hydrogen bond donors listed in the table below have a hydrogen atom that is not involved in a hydrogen bond in the optimized hydrogen bond network.

Hydrogen bond donors that are buried inside the protein normally use all of their hydrogens to form hydrogen bonds within the protein. If there are any non hydrogen bonded buried hydrogen bond donors in the structure they will be listed here. In very good structures the number of listed atoms will tend to zero.

Waters are not listed by this option.

   8 GLN   (   8-)  A      N
  10 ARG   (  10-)  A      NH1
  59 TRP   (  59-)  A      N
  67 TYR   (  67-)  A      OH
  87 ASN   (  87-)  A      ND2
 101 HIS   ( 101-)  A      N
 138 ASP   ( 138-)  A      N
 142 LYS   ( 142-)  A      N
 164 GLY   ( 164-)  A      N
 193 GLY   ( 193-)  A      N
 273 LYS   ( 273-)  A      N
 281 GLY   ( 281-)  A      N
 295 PHE   ( 295-)  A      N
 316 TRP   ( 316-)  A      N
 316 TRP   ( 316-)  A      NE1
 337 ARG   ( 337-)  A      NH2
 342 TYR   ( 342-)  A      N
 344 TRP   ( 344-)  A      N
 357 TRP   ( 357-)  A      N
 369 GLU   ( 369-)  A      N
 375 ASP   ( 375-)  A      N
 434 TRP   ( 434-)  A      N
Only metal coordination for  100 ASN  ( 100-) A      OD1

Warning: Buried unsatisfied hydrogen bond acceptors

The buried side-chain hydrogen bond acceptors listed in the table below are not involved in a hydrogen bond in the optimized hydrogen bond network.

Side-chain hydrogen bond acceptors buried inside the protein normally form hydrogen bonds within the protein. If there are any not hydrogen bonded in the optimized hydrogen bond network they will be listed here.

Waters are not listed by this option.

  15 HIS   (  15-)  A      NE2
 272 GLU   ( 272-)  A      OE1

Warning: Unusual ion packing

We implemented the ion valence determination method of Brown and Wu [REF] similar to Nayal and Di Cera [REF]. See also Mueller, Koepke and Sheldrick [REF]. It must be stated that the validation of ions in PDB files is very difficult. Ideal ion-ligand distances often differ no more than 0.1 Angstrom, and in a 2.0 Angstrom resolution structure 0.1 Angstrom is not very much. Nayal and Di Cera showed that this method has great potential, but the method has not been validated. Part of our implementation (comparing ion types) is even fully new and despite that we see it work well in the few cases that are trivial, we must emphasize that this validation method is untested. See: swift.cmbi.ru.nl/teach/theory/ for a detailed explanation.

The output gives the ion, the valency score for the ion itself, the valency score for the suggested alternative ion, and a series of possible comments *1 indicates that the suggested alternate atom type has been observed in the PDB file at another location in space. *2 indicates that WHAT IF thinks to have found this ion type in the crystallisation conditions as described in the REMARK 280 cards of the PDB file. *S Indicates that this ions is located at a special position (i.e. at a symmetry axis). N4 stands for NH4+.

 499  CA   ( 498-)  A     0.68   0.92 Scores about as good as NA

Warning: Unusual water packing

We implemented the ion valence determination method of Brown and Wu [REF] similar to Nayal and Di Cera [REF] and Mueller, Koepke and Sheldrick [REF]. It must be stated that the validation of ions in PDB files is very difficult. Ideal ion-ligand distances often differ no more than 0.1 Angstrom, and in a 2.0 Angstrom resolution structure 0.1 Angstrom is not very much. Nayal and Di Cera showed that this method nevertheless has great potential for detecting water molecules that actually should be metal ions. The method has not been extensively validated, though. Part of our implementation (comparing waters with multiple ion types) is even fully new and despite that we see it work well in the few cases that are trivial, we must emphasize that this method is untested.

The score listed is the valency score. This number should be close to (preferably a bit above) 1.0 for the suggested ion to be a likely alternative for the water molecule. Ions listed in brackets are good alternate choices. *1 indicates that the suggested ion-type has been observed elsewhere in the PDB file too. *2 indicates that the suggested ion-type has been observed in the REMARK 280 cards of the PDB file. Ion-B and ION-B indicate that the B-factor of this water is high, or very high, respectively. H2O-B indicates that the B-factors of atoms that surround this water/ion are suspicious. See: swift.cmbi.ru.nl/teach/theory/ for a detailed explanation.

 501 HOH   ( 585 )  A      O  0.89  K  4

Warning: Possible wrong residue type

The residues listed in the table below have a weird environment that cannot be improved by rotamer flips. This can mean one of three things, non of which WHAT CHECK really can do much about. 1) The side chain has actually another rotamer than is present in the PDB file; 2) A counter ion is present in the structure but is not given in the PDB file; 3) The residue actually is another amino acid type. The annotation 'Alt-rotamer' indicates that WHAT CHECK thinks you might want to find an alternate rotamer for this residue. The annotation 'Sym-induced' indicates that WHAT CHECK believes that symmetry contacts might have something to do with the difficulties of this residue's side chain. Determination of these two annotations is difficult, so their absence is less meaningful than their presence. The annotation Ligand-bound indicates that a ligand seems involved with this residue. In nine of ten of these cases this indicates that the ligand is causing the weird situation rather than the residue.

 236 ASP   ( 236-)  A   H-bonding suggests Asn

Final summary

Note: Summary report for users of a structure

This is an overall summary of the quality of the structure as compared with current reliable structures. This summary is most useful for biologists seeking a good structure to use for modelling calculations.

The second part of the table mostly gives an impression of how well the model conforms to common refinement restraint values. The first part of the table shows a number of global quality indicators.


Structure Z-scores, positive is better than average:

  1st generation packing quality :  -0.899
  2nd generation packing quality :  -1.586
  Ramachandran plot appearance   :  -1.347
  chi-1/chi-2 rotamer normality  :  -2.300
  Backbone conformation          :  -1.298

RMS Z-scores, should be close to 1.0:
  Bond lengths                   :   0.397 (tight)
  Bond angles                    :   0.698
  Omega angle restraints         :   0.460 (tight)
  Side chain planarity           :   0.241 (tight)
  Improper dihedral distribution :   0.713
  B-factor distribution          :   0.574
  Inside/Outside distribution    :   1.008

Note: Summary report for depositors of a structure

This is an overall summary of the quality of the X-ray structure as compared with structures solved at similar resolutions. This summary can be useful for a crystallographer to see if the structure makes the best possible use of the data. Warning. This table works well for structures solved in the resolution range of the structures in the WHAT IF database, which is presently (summer 2008) mainly 1.1 - 1.3 Angstrom. The further the resolution of your file deviates from this range the more meaningless this table becomes.

The second part of the table mostly gives an impression of how well the model conforms to common refinement restraint values. The first part of the table shows a number of global quality indicators, which have been calibrated against structures of similar resolution.

Resolution found in PDB file : 2.00


Structure Z-scores, positive is better than average:

  1st generation packing quality :  -0.6
  2nd generation packing quality :  -1.1
  Ramachandran plot appearance   :  -0.7
  chi-1/chi-2 rotamer normality  :  -1.3
  Backbone conformation          :  -1.4

RMS Z-scores, should be close to 1.0:
  Bond lengths                   :   0.397 (tight)
  Bond angles                    :   0.698
  Omega angle restraints         :   0.460 (tight)
  Side chain planarity           :   0.241 (tight)
  Improper dihedral distribution :   0.713
  B-factor distribution          :   0.574
  Inside/Outside distribution    :   1.008
==============

WHAT IF
    G.Vriend,
      WHAT IF: a molecular modelling and drug design program,
    J. Mol. Graph. 8, 52--56 (1990).

WHAT_CHECK (verification routines from WHAT IF)
    R.W.W.Hooft, G.Vriend, C.Sander and E.E.Abola,
      Errors in protein structures
    Nature 381, 272 (1996).
    (see also http://swift.cmbi.ru.nl/gv/whatcheck for a course and extra inform

Bond lengths and angles, protein residues
    R.Engh and R.Huber,
      Accurate bond and angle parameters for X-ray protein structure
      refinement,
    Acta Crystallogr. A47, 392--400 (1991).

Bond lengths and angles, DNA/RNA
    G.Parkinson, J.Voitechovsky, L.Clowney, A.T.Bruenger and H.Berman,
      New parameters for the refinement of nucleic acid-containing structures
    Acta Crystallogr. D52, 57--64 (1996).

DSSP
    W.Kabsch and C.Sander,
      Dictionary of protein secondary structure: pattern
      recognition of hydrogen bond and geometrical features
    Biopolymers 22, 2577--2637 (1983).

Hydrogen bond networks
    R.W.W.Hooft, C.Sander and G.Vriend,
      Positioning hydrogen atoms by optimizing hydrogen bond networks in
      protein structures
    PROTEINS, 26, 363--376 (1996).

Matthews' Coefficient
    B.W.Matthews
      Solvent content of Protein Crystals
    J. Mol. Biol. 33, 491--497 (1968).

Protein side chain planarity
    R.W.W. Hooft, C. Sander and G. Vriend,
      Verification of protein structures: side-chain planarity
    J. Appl. Cryst. 29, 714--716 (1996).

Puckering parameters
    D.Cremer and J.A.Pople,
      A general definition of ring puckering coordinates
    J. Am. Chem. Soc. 97, 1354--1358 (1975).

Quality Control
    G.Vriend and C.Sander,
      Quality control of protein models: directional atomic
      contact analysis,
    J. Appl. Cryst. 26, 47--60 (1993).

Ramachandran plot
    G.N.Ramachandran, C.Ramakrishnan and V.Sasisekharan,
      Stereochemistry of Polypeptide Chain Conformations
    J. Mol. Biol. 7, 95--99 (1963).

Symmetry Checks
    R.W.W.Hooft, C.Sander and G.Vriend,
      Reconstruction of symmetry related molecules from protein
      data bank (PDB) files
    J. Appl. Cryst. 27, 1006--1009 (1994).

Ion Checks
    I.D.Brown and K.K.Wu,
      Empirical Parameters for Calculating Cation-Oxygen Bond Valences
    Acta Cryst. B32, 1957--1959 (1975).

    M.Nayal and E.Di Cera,
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