WHAT IF Check report

This file was created 2012-01-31 from WHAT_CHECK output by a conversion script. If you are new to WHAT_CHECK, please study the pdbreport pages. There also exists a legend to the output.

Please note that you are looking at an abridged version of the output (all checks that gave normal results have been removed from this report). You can have a look at the Full report instead.

Verification log for pdb2sil.ent

Non-validating, descriptive output paragraph

Note: Ramachandran plot

In this Ramachandran plot x-signs represent glycines, squares represent prolines, and plus-signs represent the other residues. If too many plus- signs fall outside the contoured areas then the molecule is poorly refined (or worse). Proline can only occur in the narrow region around phi=-60 that also falls within the other contour islands.

In a colour picture, the residues that are part of a helix are shown in blue, strand residues in red. Preferred regions for helical residues are drawn in blue, for strand residues in red, and for all other residues in green. A full explanation of the Ramachandran plot together with a series of examples can be found at the WHAT_CHECK website.

Chain identifier: A

Coordinate problems, unexpected atoms, B-factor and occupancy checks

Warning: What type of B-factor?

WHAT IF does not yet know well how to cope with B-factors in case TLS has been used. It simply assumes that the B-factor listed on the ATOM and HETATM cards are the total B-factors. When TLS refinement is used that assumption sometimes is not correct. TLS seems not mentioned in the header of the PDB file. But anyway, if WHAT IF complains about your B-factors, and you think that they are OK, then check for TLS related B-factor problems first.

Obviously, the temperature at which the X-ray data was collected has some importance too:

Temperature cannot be read from the PDB file. This most likely means that the temperature is listed as NULL (meaning unknown) in the PDB file.

Error: The B-factors of bonded atoms show signs of over-refinement

For each of the bond types in a protein a distribution was derived for the difference between the square roots of the B-factors of the two atoms. All bonds in the current protein were scored against these distributions. The number given below is the RMS Z-score over the structure. For a structure with completely restrained B-factors within residues, this value will be around 0.35, for extremely high resolution structures refined with free isotropic B-factors this number is expected to be near 1.0. Any value over 1.5 is sign of severe over-refinement of B-factors.

RMS Z-score : 1.962 over 2583 bonds
Average difference in B over a bond : 4.03
RMS difference in B over a bond : 6.82

Note: B-factor plot

The average atomic B-factor per residue is plotted as function of the residue number.

Chain identifier: A

Nomenclature related problems

Warning: Arginine nomenclature problem

The arginine residues listed in the table below have their N-H-1 and N-H-2 swapped.

  89 ARG   (  90-)  A
 250 ARG   ( 251-)  A

Geometric checks

Warning: Unusual bond lengths

The bond lengths listed in the table below were found to deviate more than 4 sigma from standard bond lengths (both standard values and sigmas for amino acid residues have been taken from Engh and Huber [REF], for DNA they were taken from Parkinson et al [REF]). In the table below for each unusual bond the bond length and the number of standard deviations it differs from the normal value is given.

Atom names starting with "-" belong to the previous residue in the chain. If the second atom name is "-SG*", the disulphide bridge has a deviating length.

 198 LYS   ( 199-)  A      CE   NZ    1.64    5.0
 366 GLU   ( 367-)  A      CD   OE2   1.33    4.4

Warning: Unusual bond angles

The bond angles listed in the table below were found to deviate more than 4 sigma from standard bond angles (both standard values and sigma for protein residues have been taken from Engh and Huber [REF], for DNA/RNA from Parkinson et al [REF]). In the table below for each strange angle the bond angle and the number of standard deviations it differs from the standard values is given. Please note that disulphide bridges are neglected. Atoms starting with "-" belong to the previous residue in the sequence.

   1 THR   (   2-)  A      C    CA   CB  126.64    8.7
   2 VAL   (   3-)  A      N    CA   C    94.49   -6.0
   3 GLU   (   4-)  A     -O   -C    N   115.21   -4.9
   3 GLU   (   4-)  A     -CA  -C    N   127.75    5.8
   9 LYS   (  10-)  A      CG   CD   CE  121.59    4.5
  34 TYR   (  35-)  A      CA   CB   CG  121.26    4.0
  36 ARG   (  37-)  A      CB   CG   CD  101.62   -6.2
  36 ARG   (  37-)  A      CD   NE   CZ  129.51    4.3
  36 ARG   (  37-)  A      NE   CZ   NH1 134.62    7.7
  36 ARG   (  37-)  A      NE   CZ   NH2 108.10   -6.3
  45 LYS   (  46-)  A      CA   CB   CG  104.75   -4.7
  53 ASP   (  54-)  A      C    CA   CB  102.31   -4.1
  58 THR   (  59-)  A      C    CA   CB   94.15   -8.4
  58 THR   (  59-)  A      CA   CB   OG1 117.20    5.1
  58 THR   (  59-)  A      CG2  CB   OG1 117.53    4.1
  61 ASP   (  62-)  A      CA   CB   CG  120.41    7.8
  71 ARG   (  72-)  A      CG   CD   NE  101.21   -5.5
  77 LYS   (  78-)  A      CA   CB   CG  104.36   -4.9
  89 ARG   (  90-)  A      CG   CD   NE   90.90  -11.5
  89 ARG   (  90-)  A      CD   NE   CZ  146.69   13.9
  89 ARG   (  90-)  A      NE   CZ   NH1 135.87    8.4
  89 ARG   (  90-)  A      NE   CZ   NH2 105.43   -7.7
  91 ASN   (  92-)  A      N    CA   CB  102.56   -4.7
  97 VAL   (  98-)  A      N    CA   CB  117.74    4.3
  99 ASP   ( 100-)  A     -C    N    CA  128.97    4.0
And so on for a total of 80 lines.

Error: Nomenclature error(s)

Checking for a hand-check. WHAT IF has over the course of this session already corrected the handedness of atoms in several residues. These were administrative corrections. These residues are listed here.

  89 ARG   (  90-)  A
 250 ARG   ( 251-)  A

Warning: Chirality deviations detected

The atoms listed in the table below have an improper dihedral value that is deviating from expected values. As the improper dihedral values are all getting very close to ideal values in recent X-ray structures, and as we actually do not know how big the spread around these values should be, this check only warns for 6 sigma deviations.

Improper dihedrals are a measure of the chirality/planarity of the structure at a specific atom. Values around -35 or +35 are expected for chiral atoms, and values around 0 for planar atoms. Planar side chains are left out of the calculations, these are better handled by the planarity checks.

Three numbers are given for each atom in the table. The first is the Z-score for the improper dihedral. The second number is the measured improper dihedral. The third number is the expected value for this atom type. A final column contains an extra warning if the chirality for an atom is opposite to the expected value.

Please also see the previous table that lists a series of administrative chirality problems that were corrected automatically upon reading-in the PDB file.

   1 THR   (   2-)  A      CA   -11.1    15.34    33.84
  58 THR   (  59-)  A      CA     6.8    45.19    33.84
 115 VAL   ( 116-)  A      CB    14.5   -13.90   -32.96
 143 LEU   ( 144-)  A      CG     8.6   -17.92   -33.01
 158 THR   ( 159-)  A      CA     7.8    46.80    33.84
 279 VAL   ( 280-)  A      CB    20.8    -5.73   -32.96
 323 VAL   ( 324-)  A      CB     9.1   -21.04   -32.96
 357 VAL   ( 358-)  A      CB    10.1   -19.79   -32.96
 358 VAL   ( 359-)  A      CB     7.9   -22.65   -32.96
The average deviation= 1.868

Error: Tau angle problems

The side chains of the residues listed in the table below contain a tau angle (N-Calpha-C) that was found to deviate from te expected value by more than 4.0 times the expected standard deviation. The number in the table is the number of standard deviations this RMS value deviates from the expected value.

   2 VAL   (   3-)  A    6.01
 240 LEU   ( 241-)  A    4.78
 229 SER   ( 230-)  A    4.33
 239 SER   ( 240-)  A    4.22

Warning: High tau angle deviations

The RMS Z-score for the tau angles (N-Calpha-C) in the structure is too high. For well refined structures this number is expected to be near 1.0. The fact that it is higher than 1.5 worries us. However, we determined the tau normal distributions from 500 high-resolution X-ray structures, rather than from CSD data, so we cannot be 100 percent certain about these numbers.

Tau angle RMS Z-score : 1.531

Torsion-related checks

Warning: Torsion angle evaluation shows unusual residues

The residues listed in the table below contain bad or abnormal torsion angles.

These scores give an impression of how `normal' the torsion angles in protein residues are. All torsion angles except omega are used for calculating a `normality' score. Average values and standard deviations were obtained from the residues in the WHAT IF database. These are used to calculate Z-scores. A residue with a Z-score of below -2.0 is poor, and a score of less than -3.0 is worrying. For such residues more than one torsion angle is in a highly unlikely position.

 258 PHE   ( 259-)  A    -3.1
 135 PRO   ( 136-)  A    -2.9
  37 ILE   (  38-)  A    -2.8
 181 LEU   ( 182-)  A    -2.7
  58 THR   (  59-)  A    -2.5
 380 TYR   ( 381-)  A    -2.5
 292 LEU   ( 293-)  A    -2.4
 331 PRO   ( 332-)  A    -2.4
 279 VAL   ( 280-)  A    -2.3
 227 PHE   ( 228-)  A    -2.2
 147 THR   ( 148-)  A    -2.2
 133 LYS   ( 134-)  A    -2.2
 338 GLY   ( 339-)  A    -2.1
  11 GLU   (  12-)  A    -2.1
 204 LEU   ( 205-)  A    -2.1
 207 SER   ( 208-)  A    -2.0
 330 TYR   ( 331-)  A    -2.0
 143 LEU   ( 144-)  A    -2.0

Warning: Backbone evaluation reveals unusual conformations

The residues listed in the table below have abnormal backbone torsion angles.

Residues with `forbidden' phi-psi combinations are listed, as well as residues with unusual omega angles (deviating by more than 3 sigma from the normal value). Please note that it is normal if about 5 percent of the residues is listed here as having unusual phi-psi combinations.

  11 GLU   (  12-)  A  Poor phi/psi
  12 GLY   (  13-)  A  Poor phi/psi
  37 ILE   (  38-)  A  Poor phi/psi
  89 ARG   (  90-)  A  Poor phi/psi
  99 ASP   ( 100-)  A  Poor phi/psi
 108 GLN   ( 109-)  A  Poor phi/psi
 134 ALA   ( 135-)  A  PRO omega poor
 149 ASP   ( 150-)  A  Poor phi/psi
 177 VAL   ( 178-)  A  Poor phi/psi
 207 SER   ( 208-)  A  Poor phi/psi
 227 PHE   ( 228-)  A  Poor phi/psi
 229 SER   ( 230-)  A  Poor phi/psi
 238 ALA   ( 239-)  A  Poor phi/psi
 270 LYS   ( 271-)  A  Poor phi/psi
 275 ARG   ( 276-)  A  Poor phi/psi
 277 HIS   ( 278-)  A  Poor phi/psi
 305 ASP   ( 306-)  A  Poor phi/psi
 310 ASP   ( 311-)  A  Poor phi/psi
 350 VAL   ( 351-)  A  Poor phi/psi
 chi-1/chi-2 correlation Z-score : -1.989

Warning: Unusual backbone conformations

For the residues listed in the table below, the backbone formed by itself and two neighbouring residues on either side is in a conformation that is not seen very often in the database of solved protein structures. The number given in the table is the number of similar backbone conformations in the database with the same amino acid in the centre.

For this check, backbone conformations are compared with database structures using C-alpha superpositions with some restraints on the backbone oxygen positions.

A residue mentioned in the table can be part of a strange loop, or there might be something wrong with it or its directly surrounding residues. There are a few of these in every protein, but in any case it is worth looking at!

   3 GLU   (   4-)  A      0
   8 PHE   (   9-)  A      0
  10 ALA   (  11-)  A      0
  11 GLU   (  12-)  A      0
  19 LYS   (  20-)  A      0
  21 ASN   (  22-)  A      0
  28 SER   (  29-)  A      0
  34 TYR   (  35-)  A      0
  36 ARG   (  37-)  A      0
  37 ILE   (  38-)  A      0
  38 PRO   (  39-)  A      0
  45 LYS   (  46-)  A      0
  56 HIS   (  57-)  A      0
  57 ASN   (  58-)  A      0
  58 THR   (  59-)  A      0
  62 GLN   (  63-)  A      0
  63 SER   (  64-)  A      0
  64 PHE   (  65-)  A      0
  74 ASP   (  75-)  A      0
  77 LYS   (  78-)  A      0
  78 THR   (  79-)  A      0
  81 LYS   (  82-)  A      0
  82 LYS   (  83-)  A      0
  84 ALA   (  85-)  A      0
  95 SER   (  96-)  A      0
And so on for a total of 176 lines.

Warning: Omega angles too tightly restrained

The omega angles for trans-peptide bonds in a structure are expected to give a gaussian distribution with the average around +178 degrees and a standard deviation around 5.5 degrees. These expected values were obtained from very accurately determined structures. Many protein structures are too tightly restrained. This seems to be the case with the current structure too, as the observed standard deviation is below 4.0 degrees.

Standard deviation of omega values : 3.207

Warning: Unusual PRO puckering amplitudes

The proline residues listed in the table below have a puckering amplitude that is outside of normal ranges. Puckering parameters were calculated by the method of Cremer and Pople [REF]. Normal PRO rings have a puckering amplitude Q between 0.20 and 0.45 Angstrom. If Q is lower than 0.20 Angstrom for a PRO residue, this could indicate disorder between the two different normal ring forms (with C-gamma below and above the ring, respectively). If Q is higher than 0.45 Angstrom something could have gone wrong during the refinement. Be aware that this is a warning with a low confidence level. See: Who checks the checkers? Four validation tools applied to eight atomic resolution structures [REF]

 375 PRO   ( 376-)  A    0.15 LOW

Warning: Unusual PRO puckering phases

The proline residues listed in the table below have a puckering phase that is not expected to occur in protein structures. Puckering parameters were calculated by the method of Cremer and Pople [REF]. Normal PRO rings approximately show a so-called envelope conformation with the C-gamma atom above the plane of the ring (phi=+72 degrees), or a half-chair conformation with C-gamma below and C-beta above the plane of the ring (phi=-90 degrees). If phi deviates strongly from these values, this is indicative of a very strange conformation for a PRO residue, and definitely requires a manual check of the data. Be aware that this is a warning with a low confidence level. See: Who checks the checkers? Four validation tools applied to eight atomic resolution structures [REF].

 135 PRO   ( 136-)  A   -48.2 half-chair C-beta/C-alpha (-54 degrees)
 220 PRO   ( 221-)  A  -120.5 half-chair C-delta/C-gamma (-126 degrees)
 331 PRO   ( 332-)  A   -62.9 half-chair C-beta/C-alpha (-54 degrees)

Bump checks

Error: Abnormally short interatomic distances

The pairs of atoms listed in the table below have an unusually short interactomic distance; each bump is listed in only one direction.

The contact distances of all atom pairs have been checked. Two atoms are said to `bump' if they are closer than the sum of their Van der Waals radii minus 0.40 Angstrom. For hydrogen bonded pairs a tolerance of 0.55 Angstrom is used. The first number in the table tells you how much shorter that specific contact is than the acceptable limit. The second distance is the distance between the centres of the two atoms. Although we believe that two water atoms at 2.4 A distance are too close, we only report water pairs that are closer than this rather short distance.

The last text-item on each line represents the status of the atom pair. If the final column contains the text 'HB', the bump criterion was relaxed because there could be a hydrogen bond. Similarly relaxed criteria are used for 1-3 and 1-4 interactions (listed as 'B2' and 'B3', respectively). BL indicates that the B-factors of the clashing atoms have a low B-factor thereby making this clash even more worrisome. INTRA and INTER indicate whether the clashes are between atoms in the same asymmetric unit, or atoms in symmetry related asymmetric units, respectively.

 131 ARG   ( 132-)  A      NH1 <->  383 HOH   ( 626 )  A      O      0.35    2.35  INTRA
 255 THR   ( 256-)  A      CG2 <->  259 GLY   ( 260-)  A      N      0.25    2.85  INTRA BL
 372 ARG   ( 373-)  A      NE  <->  383 HOH   ( 660 )  A      O      0.23    2.47  INTRA
 158 THR   ( 159-)  A      CG2 <->  160 ILE   ( 161-)  A      N      0.22    2.88  INTRA BL
 139 TRP   ( 140-)  A      CG  <->  140 ASP   ( 141-)  A      N      0.20    2.80  INTRA BL
  82 LYS   (  83-)  A      NZ  <->  383 HOH   ( 517 )  A      O      0.15    2.55  INTRA BF
 166 LYS   ( 167-)  A      NZ  <->  383 HOH   ( 683 )  A      O      0.13    2.57  INTRA BF
 197 THR   ( 198-)  A      CB  <->  199 ASN   ( 200-)  A      ND2    0.13    2.97  INTRA
  66 ASP   (  67-)  A      OD1 <->   89 ARG   (  90-)  A      NH2    0.12    2.58  INTRA BL
 277 HIS   ( 278-)  A      N   <->  383 HOH   ( 651 )  A      O      0.11    2.59  INTRA
  40 MET   (  41-)  A      CE  <->   48 ILE   (  49-)  A      CG2    0.10    3.10  INTRA BF
 197 THR   ( 198-)  A      OG1 <->  199 ASN   ( 200-)  A      ND2    0.10    2.60  INTRA
 291 LYS   ( 292-)  A      NZ  <->  381 ASN   ( 382-)  A      O      0.08    2.62  INTRA
 286 ILE   ( 287-)  A      N   <->  293 VAL   ( 294-)  A      O      0.07    2.63  INTRA BL
 211 SER   ( 212-)  A      OG  <->  214 GLY   ( 215-)  A      N      0.06    2.64  INTRA BL
 316 HIS   ( 317-)  A      CD2 <->  323 VAL   ( 324-)  A      CG1    0.05    3.15  INTRA BL
 237 ASN   ( 238-)  A      C   <->  238 ALA   ( 239-)  A      CA     0.05    2.25  INTRA BF
 123 ASN   ( 124-)  A      ND2 <->  383 HOH   ( 680 )  A      O      0.04    2.66  INTRA
 383 HOH   ( 601 )  A      O   <->  383 HOH   ( 674 )  A      O      0.04    2.16  INTRA
  84 ALA   (  85-)  A      O   <->  383 HOH   ( 617 )  A      O      0.04    2.36  INTRA
  43 THR   (  44-)  A      O   <->   46 GLY   (  47-)  A      N      0.03    2.67  INTRA BL
 256 LYS   ( 257-)  A      N   <->  383 HOH   ( 645 )  A      O      0.03    2.67  INTRA
 251 ARG   ( 252-)  A      NE  <->  269 ASP   ( 270-)  A      OD1    0.03    2.67  INTRA BL
  55 ARG   (  56-)  A      NH1 <->   61 ASP   (  62-)  A      OD1    0.03    2.67  INTRA BL
   2 VAL   (   3-)  A      CB  <->   77 LYS   (  78-)  A      CG     0.03    3.17  INTRA BF
 108 GLN   ( 109-)  A      N   <->  383 HOH   ( 581 )  A      O      0.03    2.67  INTRA
 255 THR   ( 256-)  A      CG2 <->  261 THR   ( 262-)  A      O      0.03    2.77  INTRA
  45 LYS   (  46-)  A      N   <->   46 GLY   (  47-)  A      N      0.03    2.57  INTRA BL
 303 ASN   ( 304-)  A      N   <->  304 ASN   ( 305-)  A      N      0.03    2.57  INTRA BL
 158 THR   ( 159-)  A      CG2 <->  159 ASN   ( 160-)  A      N      0.02    2.98  INTRA BL
 216 THR   ( 217-)  A      OG1 <->  383 HOH   ( 639 )  A      O      0.02    2.38  INTRA
 199 ASN   ( 200-)  A      ND2 <->  383 HOH   ( 627 )  A      O      0.01    2.69  INTRA BF
 262 TRP   ( 263-)  A      NE1 <->  383 HOH   ( 548 )  A      O      0.01    2.69  INTRA BL
  40 MET   (  41-)  A      CE  <->   50 VAL   (  51-)  A      CG2    0.01    3.19  INTRA BF
 372 ARG   ( 373-)  A      N   <->  373 HIS   ( 374-)  A      N      0.01    2.59  INTRA BL
 105 ALA   ( 106-)  A      O   <->  112 THR   ( 113-)  A      N      0.01    2.69  INTRA BL
 381 ASN   ( 382-)  A      C   <->  382 ASN   ( 382-)  A      OXT    0.01    2.59  INTRA BF
 161 HIS   ( 162-)  A      NE2 <->  383 HOH   ( 533 )  A      O      0.01    2.69  INTRA BL
 256 LYS   ( 257-)  A      N   <->  257 ASP   ( 258-)  A      N      0.01    2.59  INTRA BL

Packing, accessibility and threading

Note: Inside/Outside RMS Z-score plot

The Inside/Outside distribution normality RMS Z-score over a 15 residue window is plotted as function of the residue number. High areas in the plot (above 1.5) indicate unusual inside/outside patterns.

Chain identifier: A

Warning: Abnormal packing environment for some residues

The residues listed in the table below have an unusual packing environment.

The packing environment of the residues is compared with the average packing environment for all residues of the same type in good PDB files. A low packing score can indicate one of several things: Poor packing, misthreading of the sequence through the density, crystal contacts, contacts with a co-factor, or the residue is part of the active site. It is not uncommon to see a few of these, but in any case this requires further inspection of the residue.

 108 GLN   ( 109-)  A      -5.94
  18 GLN   (  19-)  A      -5.86
 223 TYR   ( 224-)  A      -5.45
 306 TYR   ( 307-)  A      -5.45
 167 ASN   ( 168-)  A      -5.29
 319 TYR   ( 320-)  A      -5.24
 290 ASN   ( 291-)  A      -5.01

Warning: Abnormal packing environment for sequential residues

A stretch of at least three sequential residues with a questionable packing environment was found. This could indicate that these residues are part of a strange loop. It might also be an indication of misthreading in the density. However, it can also indicate that one or more residues in this stretch have other problems such as, for example, missing atoms, very weird angles or bond lengths, etc.

The table below lists the first and last residue in each stretch found, as well as the average residue score of the series.

  28 SER   (  29-)  A        30 - GLY     31- ( A)         -4.18

Note: Quality value plot

The quality value smoothed over a 10 residue window is plotted as function of the residue number. Low areas in the plot (below -2.0) indicate unusual packing.

Chain identifier: A

Warning: Low packing Z-score for some residues

The residues listed in the table below have an unusual packing environment according to the 2nd generation packing check. The score listed in the table is a packing normality Z-score: positive means better than average, negative means worse than average. Only residues scoring less than -2.50 are listed here. These are the unusual residues in the structure, so it will be interesting to take a special look at them.

  94 LEU   (  95-)  A   -3.48
 349 ASN   ( 350-)  A   -2.84

Note: Second generation quality Z-score plot

The second generation quality Z-score smoothed over a 10 residue window is plotted as function of the residue number. Low areas in the plot (below -1.3) indicate unusual packing.

Chain identifier: A

Water, ion, and hydrogenbond related checks

Warning: Water molecules need moving

The water molecules listed in the table below were found to be significantly closer to a symmetry related non-water molecule than to the ones given in the coordinate file. For optimal viewing convenience revised coordinates for these water molecules should be given.

The number in brackets is the identifier of the water molecule in the input file. Suggested coordinates are also given in the table. Please note that alternative conformations for protein residues are not taken into account for this calculation. If you are using WHAT IF / WHAT-CHECK interactively, then the moved waters can be found in PDB format in the file: MOVEDH2O.pdb.

 383 HOH   ( 520 )  A      O     57.89   42.75   51.14
 383 HOH   ( 523 )  A      O     47.18   38.40   24.80

Error: Water molecules without hydrogen bonds

The water molecules listed in the table below do not form any hydrogen bonds, neither with the protein or DNA/RNA, nor with other water molecules. This is a strong indication of a refinement problem. The last number on each line is the identifier of the water molecule in the input file.

 383 HOH   ( 630 )  A      O

Error: HIS, ASN, GLN side chain flips

Listed here are Histidine, Asparagine or Glutamine residues for which the orientation determined from hydrogen bonding analysis are different from the assignment given in the input. Either they could form energetically more favourable hydrogen bonds if the terminal group was rotated by 180 degrees, or there is no assignment in the input file (atom type 'A') but an assignment could be made. Be aware, though, that if the topology could not be determined for one or more ligands, then this option will make errors.

  56 HIS   (  57-)  A
 123 ASN   ( 124-)  A
 193 GLN   ( 194-)  A
 199 ASN   ( 200-)  A
 205 ASN   ( 206-)  A
 231 ASN   ( 232-)  A
 243 ASN   ( 244-)  A
 274 ASN   ( 275-)  A
 277 HIS   ( 278-)  A
 296 HIS   ( 297-)  A
 316 HIS   ( 317-)  A
 335 ASN   ( 336-)  A

Warning: Buried unsatisfied hydrogen bond donors

The buried hydrogen bond donors listed in the table below have a hydrogen atom that is not involved in a hydrogen bond in the optimized hydrogen bond network.

Hydrogen bond donors that are buried inside the protein normally use all of their hydrogens to form hydrogen bonds within the protein. If there are any non hydrogen bonded buried hydrogen bond donors in the structure they will be listed here. In very good structures the number of listed atoms will tend to zero.

Waters are not listed by this option.

  13 GLU   (  14-)  A      N
  18 GLN   (  19-)  A      N
  36 ARG   (  37-)  A      NH1
  42 THR   (  43-)  A      OG1
  87 ASN   (  88-)  A      ND2
 158 THR   ( 159-)  A      OG1
 160 ILE   ( 161-)  A      N
 162 ASP   ( 163-)  A      N
 173 MET   ( 174-)  A      N
 184 ASN   ( 185-)  A      N
 250 ARG   ( 251-)  A      NE
 255 THR   ( 256-)  A      OG1
 257 ASP   ( 258-)  A      N
 280 GLN   ( 281-)  A      N
 282 SER   ( 283-)  A      OG
 299 ALA   ( 300-)  A      N
 328 ASP   ( 329-)  A      N
 333 VAL   ( 334-)  A      N
 373 HIS   ( 374-)  A      ND1

Warning: Buried unsatisfied hydrogen bond acceptors

The buried side-chain hydrogen bond acceptors listed in the table below are not involved in a hydrogen bond in the optimized hydrogen bond network.

Side-chain hydrogen bond acceptors buried inside the protein normally form hydrogen bonds within the protein. If there are any not hydrogen bonded in the optimized hydrogen bond network they will be listed here.

Waters are not listed by this option.

 235 GLU   ( 236-)  A      OE2
 328 ASP   ( 329-)  A      OD1

Warning: No crystallisation information

No, or very inadequate, crystallisation information was observed upon reading the PDB file header records. This information should be available in the form of a series of REMARK 280 lines. Without this information a few things, such as checking ions in the structure, cannot be performed optimally.

Warning: Possible wrong residue type

The residues listed in the table below have a weird environment that cannot be improved by rotamer flips. This can mean one of three things, non of which WHAT CHECK really can do much about. 1) The side chain has actually another rotamer than is present in the PDB file; 2) A counter ion is present in the structure but is not given in the PDB file; 3) The residue actually is another amino acid type. The annotation 'Alt-rotamer' indicates that WHAT CHECK thinks you might want to find an alternate rotamer for this residue. The annotation 'Sym-induced' indicates that WHAT CHECK believes that symmetry contacts might have something to do with the difficulties of this residue's side chain. Determination of these two annotations is difficult, so their absence is less meaningful than their presence. The annotation Ligand-bound indicates that a ligand seems involved with this residue. In nine of ten of these cases this indicates that the ligand is causing the weird situation rather than the residue.

 273 ASP   ( 274-)  A   H-bonding suggests Asn; but Alt-Rotamer

Final summary

Note: Summary report for users of a structure

This is an overall summary of the quality of the structure as compared with current reliable structures. This summary is most useful for biologists seeking a good structure to use for modelling calculations.

The second part of the table mostly gives an impression of how well the model conforms to common refinement restraint values. The first part of the table shows a number of global quality indicators.


Structure Z-scores, positive is better than average:

  1st generation packing quality :   0.187
  2nd generation packing quality :  -1.676
  Ramachandran plot appearance   :  -0.673
  chi-1/chi-2 rotamer normality  :  -1.989
  Backbone conformation          :  -0.844

RMS Z-scores, should be close to 1.0:
  Bond lengths                   :   0.819
  Bond angles                    :   1.566
  Omega angle restraints         :   0.583 (tight)
  Side chain planarity           :   1.254
  Improper dihedral distribution :   1.826 (loose)
  B-factor distribution          :   1.962 (loose)
  Inside/Outside distribution    :   0.987

Note: Summary report for depositors of a structure

This is an overall summary of the quality of the X-ray structure as compared with structures solved at similar resolutions. This summary can be useful for a crystallographer to see if the structure makes the best possible use of the data. Warning. This table works well for structures solved in the resolution range of the structures in the WHAT IF database, which is presently (summer 2008) mainly 1.1 - 1.3 Angstrom. The further the resolution of your file deviates from this range the more meaningless this table becomes.

The second part of the table mostly gives an impression of how well the model conforms to common refinement restraint values. The first part of the table shows a number of global quality indicators, which have been calibrated against structures of similar resolution.

Resolution found in PDB file : 1.60


Structure Z-scores, positive is better than average:

  1st generation packing quality :   0.5
  2nd generation packing quality :  -1.5
  Ramachandran plot appearance   :  -1.1
  chi-1/chi-2 rotamer normality  :  -2.4
  Backbone conformation          :  -1.2

RMS Z-scores, should be close to 1.0:
  Bond lengths                   :   0.819
  Bond angles                    :   1.566
  Omega angle restraints         :   0.583 (tight)
  Side chain planarity           :   1.254
  Improper dihedral distribution :   1.826 (loose)
  B-factor distribution          :   1.962 (loose)
  Inside/Outside distribution    :   0.987
==============

WHAT IF
    G.Vriend,
      WHAT IF: a molecular modelling and drug design program,
    J. Mol. Graph. 8, 52--56 (1990).

WHAT_CHECK (verification routines from WHAT IF)
    R.W.W.Hooft, G.Vriend, C.Sander and E.E.Abola,
      Errors in protein structures
    Nature 381, 272 (1996).
    (see also http://swift.cmbi.ru.nl/gv/whatcheck for a course and extra inform

Bond lengths and angles, protein residues
    R.Engh and R.Huber,
      Accurate bond and angle parameters for X-ray protein structure
      refinement,
    Acta Crystallogr. A47, 392--400 (1991).

Bond lengths and angles, DNA/RNA
    G.Parkinson, J.Voitechovsky, L.Clowney, A.T.Bruenger and H.Berman,
      New parameters for the refinement of nucleic acid-containing structures
    Acta Crystallogr. D52, 57--64 (1996).

DSSP
    W.Kabsch and C.Sander,
      Dictionary of protein secondary structure: pattern
      recognition of hydrogen bond and geometrical features
    Biopolymers 22, 2577--2637 (1983).

Hydrogen bond networks
    R.W.W.Hooft, C.Sander and G.Vriend,
      Positioning hydrogen atoms by optimizing hydrogen bond networks in
      protein structures
    PROTEINS, 26, 363--376 (1996).

Matthews' Coefficient
    B.W.Matthews
      Solvent content of Protein Crystals
    J. Mol. Biol. 33, 491--497 (1968).

Protein side chain planarity
    R.W.W. Hooft, C. Sander and G. Vriend,
      Verification of protein structures: side-chain planarity
    J. Appl. Cryst. 29, 714--716 (1996).

Puckering parameters
    D.Cremer and J.A.Pople,
      A general definition of ring puckering coordinates
    J. Am. Chem. Soc. 97, 1354--1358 (1975).

Quality Control
    G.Vriend and C.Sander,
      Quality control of protein models: directional atomic
      contact analysis,
    J. Appl. Cryst. 26, 47--60 (1993).

Ramachandran plot
    G.N.Ramachandran, C.Ramakrishnan and V.Sasisekharan,
      Stereochemistry of Polypeptide Chain Conformations
    J. Mol. Biol. 7, 95--99 (1963).

Symmetry Checks
    R.W.W.Hooft, C.Sander and G.Vriend,
      Reconstruction of symmetry related molecules from protein
      data bank (PDB) files
    J. Appl. Cryst. 27, 1006--1009 (1994).

Ion Checks
    I.D.Brown and K.K.Wu,
      Empirical Parameters for Calculating Cation-Oxygen Bond Valences
    Acta Cryst. B32, 1957--1959 (1975).

    M.Nayal and E.Di Cera,
      Valence Screening of Water in Protein Crystals Reveals Potential Na+
      Binding Sites
    J.Mol.Biol. 256 228--234 (1996).

    P.Mueller, S.Koepke and G.M.Sheldrick,
      Is the bond-valence method able to identify metal atoms in protein
      structures?
    Acta Cryst. D 59 32--37 (2003).

Checking checks
    K.Wilson, C.Sander, R.W.W.Hooft, G.Vriend, et al.
      Who checks the checkers
    J.Mol.Biol. (1998) 276,417-436.